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bg01v cag egfp hesc lines  (ATCC)


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    Structured Review

    ATCC bg01v cag egfp hesc lines
    Bg01v Cag Egfp Hesc Lines, supplied by ATCC, used in various techniques. Bioz Stars score: 92/100, based on 36 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/bg01v+cag+egfp+hesc+lines/hESC+BG01V/10__1089_slash_ten__tec__2013__0646-462-16-47
    Average 92 stars, based on 36 article reviews
    bg01v cag egfp hesc lines - by Bioz Stars, 2026-10
    92/100 stars

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    Related Articles

    Expressing:

    Article Title: Efficient Generation of Human Embryonic Stem Cell-Derived Cardiac Progenitors Based on Tissue-Specific Enhanced Green Fluorescence Protein Expression
    Article Snippet: .. Supplementary Materials: Supplementary Methods hESC culture For generation of CAG promoter driven EGFP expressing HUES9-CAG-EGFP and BG01V-CAG-EGFP hESC lines the Sleeping Beauty transposon based gene delivery method was applied to transfect HUES9 hESCs (kindly provided by Dr. Douglas Melton from the Harvard University) and BG01V hESCs (from ATCC) with a plasmid containing the cDNA of the fluorescent protein EGFP, under the control of the CAG promoter. .. HUES9-CAG-EGFP and BG01V-CAG-EGFP hESCs were cultured on mitomycin-C treated mouse embryonic fibroblast (MEF) feeder cells (Millipore), in a medium consisting of Knockout DMEM (Invitrogen), supplemented with 20% Knockout Serum Replacement (Invitrogen), 2 mM glutamine, 0.1 mM nonessential amino acids, 0.1 mM β-Mercaptoethanol and 15 ng/ml recombinant human basic fibroblast growth factor (bFGF).

    Plasmid Preparation:

    Article Title: Efficient Generation of Human Embryonic Stem Cell-Derived Cardiac Progenitors Based on Tissue-Specific Enhanced Green Fluorescence Protein Expression
    Article Snippet: .. Supplementary Materials: Supplementary Methods hESC culture For generation of CAG promoter driven EGFP expressing HUES9-CAG-EGFP and BG01V-CAG-EGFP hESC lines the Sleeping Beauty transposon based gene delivery method was applied to transfect HUES9 hESCs (kindly provided by Dr. Douglas Melton from the Harvard University) and BG01V hESCs (from ATCC) with a plasmid containing the cDNA of the fluorescent protein EGFP, under the control of the CAG promoter. .. HUES9-CAG-EGFP and BG01V-CAG-EGFP hESCs were cultured on mitomycin-C treated mouse embryonic fibroblast (MEF) feeder cells (Millipore), in a medium consisting of Knockout DMEM (Invitrogen), supplemented with 20% Knockout Serum Replacement (Invitrogen), 2 mM glutamine, 0.1 mM nonessential amino acids, 0.1 mM β-Mercaptoethanol and 15 ng/ml recombinant human basic fibroblast growth factor (bFGF).

    Control:

    Article Title: Efficient Generation of Human Embryonic Stem Cell-Derived Cardiac Progenitors Based on Tissue-Specific Enhanced Green Fluorescence Protein Expression
    Article Snippet: .. Supplementary Materials: Supplementary Methods hESC culture For generation of CAG promoter driven EGFP expressing HUES9-CAG-EGFP and BG01V-CAG-EGFP hESC lines the Sleeping Beauty transposon based gene delivery method was applied to transfect HUES9 hESCs (kindly provided by Dr. Douglas Melton from the Harvard University) and BG01V hESCs (from ATCC) with a plasmid containing the cDNA of the fluorescent protein EGFP, under the control of the CAG promoter. .. HUES9-CAG-EGFP and BG01V-CAG-EGFP hESCs were cultured on mitomycin-C treated mouse embryonic fibroblast (MEF) feeder cells (Millipore), in a medium consisting of Knockout DMEM (Invitrogen), supplemented with 20% Knockout Serum Replacement (Invitrogen), 2 mM glutamine, 0.1 mM nonessential amino acids, 0.1 mM β-Mercaptoethanol and 15 ng/ml recombinant human basic fibroblast growth factor (bFGF).



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    bg01v cag egfp hesc lines  (ATCC)
    92
    ATCC bg01v cag egfp hesc lines
    Bg01v Cag Egfp Hesc Lines, supplied by ATCC, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/bg01v+cag+egfp+hesc+lines/hESC+BG01V/10__1089_slash_ten__tec__2013__0646-462-16-47
    Average 92 stars, based on 1 article reviews
    bg01v cag egfp hesc lines - by Bioz Stars, 2026-10
    92/100 stars
    		  Buy from Supplier

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